Marcelo Bielsa has a trio of players available again for the Whites trip to face the Seagulls, including key winger Raphinha.
The Brazilian missed the game at Spurs through illness, along with Rodrigo who was suffering from a minor foot problem. The pair, along with Jamie Shackleton, are among Bielsa's options again this weekend.
"Ayling will probably be able to play with the Under 23s on Monday because he is healthy now," said Bielsa during his pre-match press conference.
"Shackleton is also healthy, Raphinha and Rodrigo are also available.
"The only two players finishing their period of recovery are Koch and Bamford. Bamford has an injury in his ankle, it has been a serious injury and the evolution changes. That's why his definitive recovery is extended.
"Koch had to go for surgery. You can't be absolutely precise, that's why I always say subject to the evolution."
Leeds are hoping to come away with three points as they bid to haul themselves away from the early season top flight relegation zone.
United have won just twice this season - sitting 17th - while Graham Potter's men are ninth but haven't picked up a win in seven league games.
The Seagulls often switch formation on a weekly basis, which could see Bielsa opt for a 4-1-4-1 or a 3-3-1-3 this weekend.
Here, we take a look at how Leeds could line-up on the south coast for the tea time clash...
7. Forshaw - CM
Has been hugely impressive on his return to the line-up after his injury lay-off. He brings an element of calm to the midfield.
8. Harrison - LW/ST
Bielsa has opted to rotate his front line in and out of the striking options when they've all been fit in the absence of Bamford. A great assist last week, should keep his spot.
9. James - LW/RW/ST
The caption says it all. He could be anywhere across the front, assuming Bielsa decides to drop Gelhardt back to the bench now Raphinha and Rodrigo are fit.
10. Raphinha - RW
The star man. A huge miss last week and has to come back in - could be the difference.
11. Rodrigo - AM/ST
Has the potentially to float around as a false nine, which allows the other three wide man to shoot in and out of the middle. Would come in for Joffy, which would be expected.