The tournament sees 16 invited under-23s teams placed into their respective Northern and Southern section groups and 48 EFL clubs placed into predetermined groups prior to the draw.
The draw for round one will take place at 4.30pm on Thursday and be screened live on Sky Sports.
If there is a situation where two teams meet in the group stages for a second successive season, then the club who was at home in 2020/21 will play away in 2021/22.
All EFL clubs will play two of their three matches at home in the group stages.
Leeds went out in the first round last season after a 7-0 hammering at Accrington Stanley was followed by a 2-2 draw at Barrow and 3-0 defeat at Blackpool.
That meant United finishing bottom of Group G but Mark Jackson's side then went to win the Premier League 2 Division Two title, storming their way to promotion.
It means that Leeds will be playing in the top tier of Academy football this season of Premier League 2 Division One.
A message from the Editor:
Leeds has a fantastic story to tell - and the Yorkshire Evening Post has been rooted firmly at the heart of telling the stories of our city since 1890.
We believe in ourselves and hope you believe in us too. We need your support to help ensure we can continue to be at the heart of life in Leeds.
Subscribe to our website and enjoy unlimited access to local news and information online and on our app.
With a digital subscription, you can read more than 5 articles, see fewer ads, enjoy faster load times, and get access to exclusive newsletters and content.
Click here to subscribe.
For more details on our newspaper subscription offers click here.
Thank you Laura Collins