The 121ft (37m) green crane that currently stands within the shell of the Majestic building on City Square will be removed from the site early on Saturday morning.
It's been there since April 2018, and will take two days to fully dismantle.
The road closures:
Wellington Street will be closed at the junction of Aire Street and King Street from midnight on Friday
The road is due to reopened in the early hours of Monday morning at the latest.
Diversions will be put in place along Aire Street and Thirsk Street.
The crane arrived to assist contractors McAlpine, who are working on the former nightclub and cinema's restoration.
The Grade II-listed 1920s building has been empty since 2014, when it was extensively damaged during a fire. It is currently being converted into a seven-floor office complex with an atrium entrance and terraces overlooking Leeds Station.
Earlier this year Channel Four were announced as the building's anchor tenants and will move 300 staff to the site in summer 2020. Speculation that the broadcaster would set up their new national head office in the Majestic began last spring and was confirmed soon after. Channel Four are currently renting office space at West Gate ahead of the move.
Their three floors at the Majestic will include a broadcasting studio so that programmes can be made in Leeds.