Leeds Travel and First Bus confirmed the news on social media.
The reopening was further confirmed by First Bus who thanked passengers for their patience.
"The A6120 Broadway Road has now reopened to traffic following the earlier Police Incident." it read.
"Thanks for your patience."
The reopening of the major route will be a welcome boost to drivers with many taking to social media to report queues of over two hours.
"So traffic jams in every direction, 2 hours plus been sat in the traffic." tweeted one driver.
"Trying to go through Kirkstall, moved less then half a mile in the last hour. Complete gridlock and standstill."
There were further reports of drivers having abandoned their cars.
West Yorkshire Police earlier confirmed their attendance at the scene of a 'serious road traffic collision.'
An air ambulance was also spotted at the scene but police are yet to provide any update on the exact nature of the incident.