The performance, on Tuesday, June 26 was interrupted when the fire alarm was sounded and a full evacuation took place.
Two fire engines from Hunslet Fire Station conducted a through investigation into the cause of the alarm at the scene and no fire was detected, but they requested that the show was cancelled, according to Leeds Grand, due to the concern for public safety.
The theatre say that all ticket holders will be contacted and that they are taking the matter 'very seriously' while all scheduled performances are set to go ahead as planned.
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The statement said: "It is with sincere regret and disappointment that, due to a fire alarm at last night’s performance of Wicked, and at the request of West Yorkshire Fire and Rescue Service, the show had to be cancelled. Whilst no fire was detected, the concern for public safety was such that it was necessary to end the performance until a thorough investigation could take place.
"We apologise wholeheartedly to everyone affected. Please be assured that we are taking the matter very seriously and will be in contact with all ticket holders. We thank you for your patience in this matter.
"Please be assured all scheduled performances of Wicked are going ahead as planned."
A fire service spokesperson said: “We are very sorry for the inconvenience this may have caused to the audience however their safety is paramount to the Fire Service.
“We had satisfied ourselves that the building was safe to re-enter by 8.48pm and firefighters left the scene.”