Staff from Deloitte will be left in the dark to tackle the Leeds Abbey Dash.
A dozen runners from the Leeds office at City Square will complete the 10k race blindfolded.
They will join 8,000 others for the event on Sunday, November 20, to raise money for Age UK.
Matt Brewer, a director at Deloitte in Leeds, said: “We wanted to do something different and provide our team with more than just a physical challenge.
“I’m slightly nervous but interested to see how it will work, as it’s something that none of us have done before.
“Taking on the blindfold challenge will give our runners a snapshot of what visually impaired people and athletes have to go through.”
He added: “It also reflects our commitment to disability sport and our sponsorship of the London 2012 Olympic and Paralympic Games.”
The team will do the race as a relay and each person will have a ‘buddy runner’ without a blindfold to guide them along the way.
Visually-impaired athlete Louise Simpson from Wakefield will also be taking part in the Abbey Dash and has been giving the team tips to help them on the day.
Tom Galliano, events fundraiser at Age UK, told the Yorkshire Evening Post: “A team of blindfolded participants in the Age UK Leeds Abbey Dash is a challenge that no-one has attempted before.
“Although the Deloitte team might not be able to see the finish line, we’ll be there to give them huge cheers on the day.
“Their fundraising efforts will help us to continue our vital work to provide support to older people in the community and across the country who need it the most.
“We hope to see as many runners as possible on the day helping to raise funds for people in later life.”
There is still time to sign-up for the race.
To find out more, visit www.ageuk.org.uk/dash or call the Dash Hotline on 0800 169 8787.
To sponsor the Deloitte running team, visit: uk.virginmoneygiving.com/team/DeloitteTheBlindfoldChallenge