Otley ran out 39-27 winners over Yorkshire rivals Wharfedale in a hotly-contested match which saw a number of penalties and both sides having a player sent off.
Wharfedale had the better of the opening and led 20-12 after half an hour with Lewis Wilson and Rob Baldwin both scoring tries.
But Otley led 24-20 at half time with Tiploma Kivulu, Alex Beaumont and Jack Mackie scoring tries, and a penalty try also being awarded.
After the break a further penalty try, conversion and penalty saw Otley take a 34-20 lead.
Wharfedale’s Jimmy Bullough scored a try to narrow the gap, but after both sides had a player sent off for fighting, Otley had the last word with a late score from Jack Mackie in the 77th minute.
Harrogate went down 20-16 at Tynedale after a close encounter.
Ilkley took a narrow 26-24 win over hosts Morley in National Three (North).
Scrum-half Joe Rowntree was in great kicking form, and helped Ilkley to a 13-5 lead at half-time with two penalties and a conversion from a penalty try, while winger Tom Clough had scored Morley’s only points.
Kirk Arundale ran in on the wing for Ilkley after the break, and Rowntree kicked that conversion and two more penalties.
Morley fly-half Mark Chester scored two second-half tries and prop Ben Bell one, but Chester missed the last conversion which would’ve tied the match.
Elsewhere Cleckheaton had a big 62-0 win at Stockport, while Sandal went down 33-23 at Wirral.
Missed penalties cost Hull Ionians dear, as they were narrowly beaten 13-6 at Loughborough Students in National One.
Winger Austin Beckett put the hosts ahead with a 16th minute try, but Ionians got off the mark two minutes later with scrum-half Isaac Green kicking a penalty.
Green hit the uprights with a 26th minute missed penalty, and although he was successfully with a 36th minute effort which put the visitors briefly ahead, Loughborough retook the lead with Josh Sharp kicking a penalty on the stroke of half time to make it 8-6.
After the break Green and Sharp both missed early penalties, and the match remained close with the only other score coming from Students’ Theo Vukasinovic who scored in the corner with four minutes remaining.
Sheffield Tigers romped to a comfortable win against the National Three Midlands Champions Scunthorpe, flanker Peter Swatkins helping himself to two tries in 40-10 victory.
Centre Liam Wallace, second row Frank Wragg and wingers Henry Packard and Alex Graham also weighed in with scores as Tigers made light work of the visitors.
Sheffield, now playing in National Three Midlands, maintained their promising start with a 23-10 win at Broadstreet.
The Abbeydale Park side went ahead with tries by prop Tobi Ademakin and winger Rupert Broadley, with Joe Cribley converting the former and adding a penalty to give the visitors a 15-0 interval lead.
A try by flanker Tom Womack and a further Cribley penalty kept Sheffield clear.
Hull made it two out of two in National Three North with a 30-17 home win over Billingham in a game closer than the final score suggested.
Although they were always in front, Hull led only 13-12 at half time but their defence held tight. Tomasi Tanumi converted two penalty tries as well as a try from centre Ali Heard early in the second half.
Promoted Doncaster Phoenix, after their promising start, went down 30-15 at Huddersfield where the hosts quickly took control but were restricted to two penalties in the second half.
Promoted Bradford and Bingley scored their first North One East win but were restricted to two tries in a 25-13 home verdict over Alnwick. Malton and Norton, promoted with the Bees, are still seeking their first league points after a 34-20 home defeat by early leaders West Hartlepool.
In a match which produced 11 tries, Driffield were 41-29 winners in their derby against Pocklington at Kellythorpe. Centre Gary Stephenson sealed their win with his second try in the last moments of the game, Will Brigham converting.
Huddersfield YMCA collected a four-try bonus in their 27-20 win at Dinnington.